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Hair Loss Study Abstract: 7-Ethoxyresorufin-O-deethylase activity in human hair roots: a potential marker for toxifying species of cytochrome P-450 isozymes.
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Title
7-Ethoxyresorufin-O-deethylase activity in human hair roots: a potential marker for
toxifying species of cytochrome P-450 isozymes.
Author
Merk HF; Mukhtar H; Schutte B; Kaufmann I; Das M; Bickers DR
Address
Department of Dermatology, University of Cologne, Koeln, West Germany.
Source
Biochem Biophys Res Commun, 148: 2, 1987 Oct 29, 755-61
Abstract
Assay systems for the evaluation of carcinogen interaction with human tissues are
essential for assessing cancer risk. Human hair roots (HHR) are a readily obtainable
epithelial tissue source that have been employed for investigating inherited enzyme
activities. In this study HHR were found to possess cytochrome P-450-dependent
7-ethoxyresorufin-O-deethylase (ERD) activity which measures cytochrome P-450 isoenzymes
that are highly specific (in the order of greater than 95%) markers for the metabolic
activation of many environmental carcinogenic substances such as the polycyclic aromatic
hydrocarbons (PAHs). Topical application of PAHs (in liquor carbonis detergens) to the
scalp of human volunteers was found to enhance the activity of this enzyme in freshly
plucked hair roots. Oral and topical administration of ketoconazole to the same subjects
resulted in an appreciable (up to 73%) inhibition of detectable enzyme activity. Our data
suggest that measurement of ERD in HHR may be a useful marker for the study of toxifying
species of cytochrome P-450 isozymes in human populations.
Language of Publication
English
Unique Identifier
88076952
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